Abstract
This study demonstrates the causes and the prevention measure of the non-specific adherence phenomenon observed in the mixed cell agglutination reaction (MCAR) method using monoclonal antibodies for ABO blood grouping from various kinds of forensic samples. The phenomenon could be suppressed by the addition of both a milk protein (6% Block-Ace) and a detergent (0.3% Tween 20) into the antibody reagents, so that the causes of the phenomenon were likely to be both the electric charge potential effect and the physical absorption of the monoclonal antibodies. In this modified MCAR method, the operation time could be shortened to be about 2/3 of that of the conventional method.
