Journal of Atherosclerosis and Thrombosis
Online ISSN : 1880-3873
Print ISSN : 1340-3478
ISSN-L : 1340-3478
Original Article
Chylomicron Remnants Stimulate Release of Interleukin-1β by THP-1 Cells
Takahiro OkumuraYoshio FujiokaShinji MorimotoMiho MasaiTsuyoshi SakodaTakeshi TsujinoShin-ichiro KashiwamuraHaruki OkamuraMitsumasa Ohyanagi
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2006 Volume 13 Issue 1 Pages 38-45


Recent findings suggest that the oxidative modification of low-density lipoproteins (LDL) and an increase in triglyceride-rich lipoprotein particles including chylomicron remnants contribute to the progression of atherosclerosis, as does the inflammatory response. We therefore examined whether and how these lipoproteins affected interleukin (IL)-1β release and mRNA expression for IL-1β and IL-18 in THP-1 cells, a human monocyte cell line. Chylomicron remnants increased IL-1β release into the conditioned medium by THP-1 in a dose- and time-dependent manner. At concentrations up to 1 μg/ml, chylomicron remnants increased IL-1β release by 4-fold compared with the control. Neither native LDL nor oxidized LDL (OxLDL) significantly increased IL-1β release. Chylomicron remnants increased IL-1β mRNA expression by 3 times. Native LDL or OxLDL did not increase IL-1β mRNA, while neither these lipoproteins nor chylomicron remnants increased IL-18 mRNA. Chylomicron remnants also increased the activities of caspase-1 and nuclear factor (NF)-κB significantly, while native LDL or OxLDL did not. In conclusion, chylomicron remnants stimulated IL-1β mRNA expression and IL-1β protein production probably via caspase-1 and NF-κB activation in THP-1 cells.

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この記事はクリエイティブ・コモンズ [表示 - 非営利 - 継承 4.0 国際]ライセンスの下に提供されています。
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