Abstract
The effect of antioxidants on lipid peroxidation were investigated using cultured aortic smooth muscle cells (5 cell lines) from 5 albino rabbits.
5 μg and 10 μg/ml of vitamin E and 2, 000 ng/ml of coenzyme Q10 were used as antioxidants. As the lipid peroxide of cells was generated in the time dependent manner with cell proliferation, it remained within the cells and did not accumurate in the media. Although ethanol which is vitamin E solvent showed suppressive effect on cell proliferation, the addition of 10 μg/ml vitamin E increased it up to the control level. On the other hand, the intracellular lipid peroxide formation expressed per DNA was inhibited by 10 μg/ml vitamin E, not by 5 μg/ml. Fatty acid analysis also howed that ethanol decreased the ratio of oleate/stearate in the cells and the addition of 10 μg/ml vitamin E increased it up to the control. Coezyme Q10 showed no difference in the cell proliferation and lipid peroxide formation compared to control.
