Abstract
The secretion of apo A-I and E from primary cultured rat hepatocytes was studied. Isolated hepatocytes were prepared according to the modified method of Berry and Friend. Both apo A-I and E concentrations in culture medium were measured by specific double antibody radioimmunoassay.
During the incubation of first 24 hours, the amounts of both apoproteins secreted from hepatocytes into culture medium increased linearly in relation to time. The amounts of apo E secreted from hepatocytes were about 4 fold greater than those of apo A-I, while plasma apo A-I levels were higher than apo E.
It is proposed that primary cultured hepatocytes are useful tools for studying apoprotein synthesis.
