Abstract
To study the disturbance of metabolism of plasma lipids and apoproteins in a familial lecithin: cholesterol acyltransferase deficiency, the effects of a low fat diet on plasma lipids, apoproteins and plasma enzyme activities were investigated. The plasma apoprotein A-1 and E levels were measured by a radioimmunoassay. The apoprotein B level was determined by the method of a single radial immunodiffusion. Lipoprotein lipase and hepatic triglyceride lipase activities were measured by an immunochemical method utilizing antiserum prepared against hepatic triglyceride lipase. By the restriction of fat in diet, the amount of triglyceriderich lipoproteins were decreased. Also, plasma apoprotein E and B levels were markedly decreased, while the improvements of both lipoprotein lipase and hepatic triglyceride lipase was not observed. These results suggest that high plasma apoprotein E in the familial lecithin: cholesterol acyltransferase deficiency is strongly related to the disturbed triglyceride-rich lipoprotein metabolism in the lecithin: cholesterol acyltransferase deficient state.
