Abstract
Vitamin E was transported through lipoproteins metabolism and HDL vitamin E (HDL-E) had been suggested to be highly related with tissue or cell levels of vitamin E (for example red blood cell). The following our results coincide with the above suggestion, that the correlationship between HDL-E (0.49±0.15mg/dl) and RBC-E (2.01±0.27μg/ml packed cell) was significant in control group, then reduced RBC-E (1.63±0.22) in aged group was linked with reduced HDL-E (0.36±0.07). On the other study, when red blood cells were fractionated by ultra-centrifugation (15, 000rpm, 90min.), the relative content of vitamin E in top fraction (young RBC) was 1.213/cell or 1.128/Ht against that in bottom fraction (old RBC).
These results let us have speculation that the tissue or cell vitamin E may be exchangeable most easily with HDL-E, however, cell has its own regulating (control) mechanism, furthermore.
In this work, electrochemical detector was proved to be able to detect 100ng or more low concentration of vitamin E in HPLC system with addition of Tocol as internal standard.
