Abstract
Monoclonal antibodies directed against lipoprotein lipase (LPL) have been prepared by immunization of mice with purified bovine milk LPL. Spleen cells from the mice were fused with the SP2/O-Ag14 line of mouse myeloma cells. Ten clones producing antibodies against LPL were isolated. Cross reactivities against human post-heparin plasma LPL were tested for six clones.
1) Two clones react strongly with bovine milk LPL but react weakly with human post-heparin plasma LPL.
2) Two clones react strongly with human post-heparin plasma LPL but weakly with bovine milk LPL.
3) The remaining two clones react only weakly to both antigens.
These monoclonal antibodies will be useful for quantification of post-heparin plasma LPL and also useful for studying synthesis of LPL by human monocyte-derived macrophages.
