Abstract
We already reported the variability of serum phospholipid levels both in normal subjects and in patients suffering from vascular diseases such as diabetes mellitus, cerebral infarction, cerebral hemorrhage, and myocardial infarction. However, the method used until now was so complex and time-consuming that we used HPLC for measurement of serum choline-containing phospholipid (PL). Elution pattern of HPLC was detected separately according to their densities, i. e. chylomicron, VLDL, LDL, HDL2, HDL3, and albumin-PL. In 52 healthy males, we measured the levels of serum PL, in which total PL 193mg/dl, LDL-PL 109mg/dl, HDL2-PL 39mg/dl, HDL4-PL 32mg/dl and albumin-PL 14mg/dl were detected respectively. Single correlation were as follows; TG vs. LDL-PL, Tch vs. LDL-PL, HDL-ch vs. HDL2-PL, HDL2-PL vs. HDL3-PL were positive whereas TG vs. HDL-ch, HDL-ch vs. A. I., LDL-PL vs. HDL2-PL were negative significantly. From these results, it was supposed that HPLC was easy to handle and to detect choline-containing phospholipid separately and clearly.
