Abstract
We have studied the effects of elastase-like enzyme (ELE) on the lipid metabolism in rats, especially about lipoprotein metabolism and post heparin lipolytic activity (PHLA) in triglyceride metabolism. Lipids in plasma and liver were slightly decreased by administration of ELE. The LCAT activity in plasma was lowered to about 80% of the control value. In lipoprotein fraction, although VLDL-TG. decreased by ELE treatment, plasma post heparin lipolytic activity was not changed between the both groups. The decrease of Apo E in VLDL fraction and increase of Apo E in HDL fraction were observed in ELE group by SDSPAGE. It is considered that the increase of Ano E in HDL fraction is brought through an increase of Apo E rich HDL (nascent HDL) released from liver which comes from decrease of the LCAT activity in plasma. In isoelectric focusing patterns of Apo HDL, increased contents of Apo E3, E4 and decreased contents of Apo A-I which is activator of plasma LCAT.
These results suggest that ELE induced increase in transport of HDL into the liver together with accelerated metabolism of plasma lipoprotein.
