Abstract
Whether light scattering index of serum (S-LSI) reflect the concentration of serum lipids, apolipoproteins and lipoproteins or not has been studied.
Subjects whose serum triglyceride (TG) level was below 700mg/dl were fasted for 13 hours and blood samples were obtained.
Serum was isolated by centrifugation, separated, and diluted 100 times with physiological saline. Light scattering index of serum (S-LSI) was measured by nephelometer (Nephelotik DN2110, Kyoto Dai-ichi Kagaku Co. Ltd.), Serum lipids were measured by enzymatic methods. Serum apoprotein levels were determined by single radial immunodiffusion method. Kits of apo A-I, A-II, E, C-II and C-III were purchased from Dai-ichi Kagaku Co. Ltd., and a kit of apo B was purchased from Hechist Co. Ltd. Serum lipoproteins were separated by sequential ultracentrifugation according to Havel's method. Lipoprotein concentrations were determined by totaling triglyceride, cholesterol and phospholipid contents in each lipoprotein fractions.
S-LSI was found to significantly correlated with serum triglyceride (r=0.94, p<0.001) and with serum phospholipid (r=0.47, p<0.001).
No correlation was observed between S-LSI and serum cholesterol (Fig. 1). A clear correlation between S-LSI and apo C-II (r=0.61, p<0.001), apo C-III (r=0.52, p<0.001) and apo E (r=0.48, p<0.001) was observed. On the other hand, no correlation was found with S-LSI and apo A-I, apo A-II and apo B (Fig. 2). There was a significant correlation observed between S-LSI and VLDL (r=0.90, p<0.001). There was a weak relationship found between S-LSI and LDL (r=0.41, p<0.05) as well as between S-LSI and HDL2 (r=0.48, p<0.01) and S-LSI and HDL3 (r=0.41, p<0.05, Fig. 3).
It has been concluded that S-LSI reflects the concentrations of serum TG, apo C-II, apo C-III and VLDL.
