Abstract
1) We developed a new technique for detecting lipid peroxide (LPO) in lipoprotein fractions. For LPO detection, MCDP method was used. For separation of lipoprotein fraction, high-performance liquid chromatography (HPLC) was used.
2) In this study, we investigated the effect of antioxidants upon the distributions of LPO in modified serum. To increase the content of LPO, we stored sera for 8 weeks at -40°C. For antioxidants, Vitamin C (10-4M) and γ-oryzanol (500μg/ml) were selected.
3) By storage, total LPO content increased, whereas antioxidants reduced such increase.
4) By HPLC, in the stored serum, an additional peak of LPO was observed, which was thought to be located in LDL-fraction. Antioxidants reduced the height of the additional peak, but not that of the original peak.
