Abstract
In many previous reports, evidences have been presented that plasma low density lipoprotein may be a major source of lipids in the naturally occurring atherosclerotic plaque. The well established Coon's fluorescient antibody technique has been employed to identify native tissue antigens in study of arterial lipoprotein.
Specific antibody against serum low density lipoprotein, labelled with f luorescin, has been used as a histochemical stain to demonstrate the presence of the lipoprotein in the arterial wall. Histological materials from normal and atheromatous aortas and large arteries has been obtained from the 1% cholesterol fed rabbits and the human subjects autopsied with 2-6 hours after the death.
Results obtained are as follows: No immunofluorescin of serum low density lipoprotein in the aortic wall were observed in the normal rabbits. On the contrary, in the cholesterol fed rabbits, fluorescin were observed within intima, media and adventitial tissues, indicating insulation of the serum lipoprotein from the lumen through the wall, then into the surrounding tissues. However, quantity of accumulated lipoprotein inside the arteries appeared in proportion to the extent and duration of hyperlipidemia.
In the all of the materials obtained from human subjects, fluorescin of the lipoprotein observed inside the arterial wall. And degree of the lipoprotein accumulation inside the aortic wall seems in proportion to the age of the subjects.
In the atheromatous section of human subjects, striking accumulation of the anti-lipoprotein fluorescin existed arround the surrounded tissues of the plaque.
