Abstract
Lipoprotein farction was made visible by polyanion precipitation in the 1% agarose gels after electrophoresis, according to the method descreibed by Wieland and Seidel (Clin. Chem. 19, 1139 (1973)). The electrophoresed samples were placed for 30min. into a bath of 0.1mol/liter MgCl2 containing 1g/liter sodium heparin and 10g/liter NaCl (solution 1), to selectively precipitate the lipoprotein band corresponding to VLDL fraction, or into a bath of 0.2mol/liter CaCl2 containing 6g/liter sodium dextran sulphate to recipitate all plasma lipoproteins (solution 2).
It was possible by this method to differenciate type III and IV. The hypoalphalipoproteinemic serum showed the almost same intensity of precipitin band in the alpha position as control.
From the results of immunoelectrophoresis, it was found that the protein precipitated by solution 2 contained not only lipoproteins but also albumin, IgG and other serum proteins.
