A Study on metabolism, secretion and excretion of glycoproteins in arteriosclerosis

Source of low-molecular binding hexoses in urine

Abstract

The relationship of urinary low-molecular binding hexoses to hypertension or diabetes has been reported by our clinic.
The binding hexoses in urine were fractionated into FrI, FrII and FrIII by Sephadex G-100 column chromatography. The molecular weight of the three fractions was in the order of FrI>FrII>FrIII. Though there was much low-molecular binding hexose (FrIII) in urine, there was little in serum and blood corpuscles.
By the column chromatography, the FrIII was demonstrated as a clear peak in the extraction of the renal cortex, but only as an unclear one in that of the renal medulla.
The FrIII of urine or renal cortex obtained by Sephadex G-100 column chromatography was then divided into four fractions by Sephadex G-15 column rechromatography, the patterns of which were similar in both materials.
The molecular weight of the first peak in the Sephadex G-15 chromatography was thought slightly larger than that of raffinose.
It is concluded that the renal cortex seems to be one of the main sources of the FrIII in urine.