1978 Volume 6 Issue 1 Pages 43-48
Using 20 microliter of serum, cholesterol concentration in each lipoprotein separated with agarose gel electrophoresis were determined by gas liquid chromatography. Procedure: After performing electrophoresis, alpha and beta (involved prebeta) lipoproteins were detected with Seidel's solution 2. Each portion was taken off and saponified. Heptan extracted cholesterol was trimethyl silanized together with beta-sitosterol added as internal standard. Result: Firstly, this method was compared with classical ultracentrifugation method analysis for the separation of each lipoprotein cholesterol and confirmed to be highly correlated each other. By the presented procedure in normal subjects (n=28), alpha cholesterol was 52±19mg/dl (M±SD) and beta+prebeta cholesterol was 113±28mg/dl. The ratio of cholesterol in beta+prebeta to alpha was 2.6±1.5. Alpha cholesterol decreased in cases with history of myocardial infarction. Beta+prebeta cholesterol highly increased in essential hyperlipidemias, diabetes mellitus, hypertensive disease, nephrotic syndrome and myocardial infarction. In 5 cases of type IIa hyperlipidemia treated with antihyperlipidemic agents, alpha cholesterol decreased about a month later paralleled with a decrease of total serum cholesterol. Assuming alpha cholesterol value as a factor of anti-atherogeneity, it should be noted that alpha cholesterol must be followed routinely together with total serum cholesterol, particularly in these treated patients. And the reported method must be useful owing to its accuracy, easiness and “not” time and money consuming.
