Abstract
A newly established immuno-precipitation using the specific goat antiserum (purified γ-globoline fraction of it) against human β-lipoprotein was applied to the fractionation of lipoprotein in human serum. The lipids in α-lipoprotein fraction, cholesterol (α-LPC), glyceride (α-LPG) and phospholipid (α-LPPL) were determined by the enzymic methods for each sort of lipids, respectively. Contents of the lipids in serum were also determined by the same methods (TC, TG and PL), and the lipid contents in β-lipoprotein fraction were calculated (β-LPC, β-LPG and β-LPPL).
Results on the contents of the lipids, fractionated, in serum of myocardial infarction are as follows.
I) Comparison between the lipids of acute myocardial infarction and those of lipid matched controls:
α-LPC and α-LPPL at the acute stage of myocardial infarction were lower than those of lipid matched controls. α-LPG, on the contrary, was elevated at the acute stage.
II) Comparison of the acute and convalescent stage:
α-LPC and α-LPPL at the convalescent stage were increased as compared with those at the acute stage. As far as α-LPG concerned, there exist no remarkable differences between acute and convalescent stage; but the elevation of share of glyceride in α-fraction was evidently demonstrated (α-LPG/TG).
III) Difference of distribution of each sort of the lipids between acute and convalescent stage:
The elevation of share of lipids in α-fraction (α-LPC/TC, α-LPG/TG and α-LPPL/PL) was clearly observed at the convalescent stage compared with those at the acute stage; almost the same degree of elevation in share of each sort of lipids was noticeable. On the contrary, the distinct but not the same degree of decline in share of each sort of the lipids was observed in β-fraction at the reconvalescence; β-LPG was markedly declined (34.6%), followed by β-LPPL (33.0%) and β-LPC (8.9%).
