Abstract
Cholesterol-oleate-14C was incorporated into the LDL by the method of Krieger. Using LDL-14C as a substrate the hydrolysis of cholesterol-oleate was investigated by the rat arterial wall homogenate. Maximum hydrolysis was observed at around pH 4.5 and the hydrolysis was time dependently increased. Cholesterol oleate hydrolysis in the acetylated LDL-14C was maximum at around pH 4.5. These results suggest that metabolic pathway was almost the same between nature and denatured LDL in the arterial wall. Furtheremore, cholesterol-oleate hydrolysis in glutaraldehyde, MDA treated, platelet aggregation-treated LDLs was investigated. The relationship between lipid acumulation and denatured LDL was discussed.
