Lipoprotein lipase (LPL) was purified from bovine milk using heparin-Sepharose 4B column Chromatography. About 60% of LPL activity was decreased for 4hr. at 30°C but the addition of Dentran sulfate (DS) did not decrease the activity. DS increased the hydrolysis of VLDL-TG by LPL. From the results using BioGel A-5m, DS bound to the VLDL-LPL complex and it was suggested that VLDL-TG hydrolysis was enhanced by the formation of VLDL-LPL-DS complex.
