Journal of Atherosclerosis and Thrombosis
Online ISSN : 1880-3873
Print ISSN : 1340-3478
ISSN-L : 1340-3478
Effects of Lysosomal Protease Inhibitors on the Degradation of Acetylated Low Density Lipoprotein in Cultured Rat Peritoneal Macrophages
Takeo KurodaMototaka YoshinariKen OkamuraKaeko OkazawaHiroshi IkenoueKaori SatoMasatoshi Fujishima
Author information
JOURNAL FREE ACCESS

1994 Volume 1 Issue 1 Pages 41-44

Details
Abstract
The effect of protease inhibitors, leupeptin and pepstatin A, on the metabolism of acetylated low density lipoprotein (acetyl-LDL) was investigated in cultured rat peritoneal macrophages and compared with that of chloroquine. While both leupeptin and pepstatin inhibited the proteolytic degradation of 125I-acetyl-LDL, a combination of both showed an additive effect. Similar to chloroquine, both protease inhibitors diminished [3H] oleate incorporation into cellular cholesteryl [3H] oleate and increased cholesterol content of macrophages. These results suggest that both thiol protease and cathepsin D participate in the physiological degradation of apolipoprotein in macrophages. The inhibition of apolipoprotein degradation seemed to have an effect on cholesterol metabolism in macrophages cultured with acetyl-LDL.
Content from these authors
© Japan Atherosclerosis Society
Previous article Next article
feedback
Top