A Monoclonal Antibody-based Enzyme Immunoassay for the Measurement of Native and Glycated Apolipoprotein B-containing Particles

Abstract

We describe the development of sandwich enzyme-linked immunosorbent assays designed to measure native and glycated apolipoprotein B-containing particles in plasma. The assays utilize monoclonal antibodies anti native or glycated apo B-LDL for coating and a polyclonal anti apoB-LDL-peroxidase conjugate as the detecting antibody. The method is specific, sensitive and precise. The intra-assay coefficient of variation for the plasma native and glycated apolipoprotein B-containing particles was determine to be 7.8% and 7.5%, respectively. The method described can provide specific and reproducible determinations of apoB and glycated-apoB containing particles in plasma it will be of great interest in the evaluation of atherosclerotic risk in dyslipoproteinemic states in diabetic and nondiabetic subjects.J Atheroscler Thromb, 1997 ; 4 : 45-49.