Abstract
In recent years, organoids have been generated from various organs with the established iPS. However, the formation of capillary, which is the key renal organoid to hemofiltration, has not been successful yet. In this study, we investigated whether nephrons can be formed by introducing the microgravity environment combined with transplantation technology.
Firstly, developing mouse kidney cells were cultured in a microgravity apparatus to form the fused spheroids. The formation of ureteric buds, nephron precursor and glomerular-like tissues were confirmed in the fused spheroids. Subsequently, the fused spheroids were transplanted under the mouse kidney capsule, which resulted in the formation of nephron-like tissue structure. Furthermore, the perfusion of fluorescent labeled albumin into the transplanted mouse showed albumin existing in the glomerular-like structure of the transplanted spheroid, and capillary structure revealed.
