Abstract
It is known that the preponderance of the antiprotease (protease inhibitor) in the circulating blood is related to the high concentration of α2-macroglobulin and α1-antitrypsin consisting of glycoprotein.
In the previous reports, however, we reported that the fibrinolytic activity was owing to high activity of plasminogen activator existing in the tracheobronchial secretion of rats, and then we speculated that protease inhibitor might not exist in it. In this paper, the electrophoretic analysis of the secretion was procedured in order to clarify the absence of protease inhibitor. Using the polyacrylamide gel disc electrophoresis, three bands were identified as albumin and globulins by protein staining of Amidoblack. Furthermore, PAS staining of polyacrylamide gel revealed the existence of glycoprotein in plasma but PAS positive substance did not exist in the secretion. From these results, it was clarified that protease inhibitor consisting of glycoprotein, namely PAS positive substance such as α2-macroglobulin did not exist in the secretion.
