Search for pterin-binding protein from Euglena

Junko Takeda; Minori Nakashima; Hiroshi Ueno; Tomoko Mori; Mineo Iseki; Masakatsu Watanabe

doi:10.14533/jbm.13.13

Junko Takeda , Minori Nakashima, Hiroshi Ueno, Tomoko Mori, Mineo Iseki, Masakatsu Watanabe

Author information

Keywords: Biopterin binding protein, biopterin sensor chip for BIAcore, organic synthesis of primary anino group to biopterin

JOURNAL OPEN ACCESS

2013 Volume 13 Issue 1 Pages 13-20

DOI https://doi.org/10.14533/jbm.13.13

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Abstract

BIAcore analysis with a biopterin (BP)-bound sensor chip could be a powerful tool for the search of pterin-binding proteins. Although preparation of the sensor chip is crucial, a conventional amine coupling procedure is not applicable for the ligand such as BP that has no primary amino group. In this study, we describe the method to introduce the primary amino group onto BP molecule efficiently.

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