1990 Volume 23 Issue 3 Pages 259-269
Affinity chromatography or affinity adsorption is an effective isolation or purification operation based on biospecific interaction, that is, biological recognition. Operations for preparative purposes have recently been utilized as a potential means of downstream processing in bioproduct manufacturing processes. For affinity chromatography and biospecific adsorption, adsorption systems of a mobile phase and a stationary adsorbent phase, the theories of mass transport between phases, the operation steps of adsorption and elution, and practical application in bio-industries are described. Other biospecific separations such as affinity partition and affinity precipitation are also described.