Continuous Separation and Concentration of Proteins Using an Annular Chromatograph

Abstract

A continuous rotating annular chromatograph with rotating feed nozzles and product collectors was used to separate two kinds of proteins, (myoglobin and hemoglobin). Simultaneously, both proteins could be concentrated by using two kinds of eluents. The cation exchange resin, CM-Sepharose CL-6B was used as an adsorbent. The adsorption forces of both proteins on CM-Sepharose CL-6B were dependent on the value of pH in the buffer solution. Therefore, both proteins could be separated and concentrated by changing pH in the eluent. Experimental results could be predicted by numerical solutions.