1992 Volume 25 Issue 6 Pages 723-728
Biocatalyst immobilization carrier was coated with calcium alginate gel by a two-step preparation procedure for prevention of leakage of the biocatalyst from the carrier. A uniform gel coating film could be formed around not only calcium alginate get beads but also chitosan beads. The thickness of calcium alginate gel coating increased with decreasing sodium alginate concentration and stirring rate, and with increasing concentration of calcium ion and diameter of the core bead. The volume of gel coating film was shown to be proportional to the mass of calcium ion initially contained in the core bead. It was also found that the thickness of gel coating film could be controlled by adjusting the calcium concentration in the core bead.