Enzyme Release Kinetics in a Cell Disruption Chamber of a Bead Mill

Ariel V. Melendres; Hiroyuki Honda; Naohiro Shiragami; Hajime Unno

doi:10.1252/jcej.26.148

Original Papers

Enzyme Release Kinetics in a Cell Disruption Chamber of a Bead Mill

Ariel V. Melendres, Hiroyuki Honda, Naohiro Shiragami, Hajime Unno

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Keywords: Biochemical Engineering, Cell Disruption, Enzyme Release, Two-Step Process, Bead Mill, Cell Wall, Cytoplasm, Membrane-Bound Enzyme

JOURNAL FREE ACCESS

1993 Volume 26 Issue 2 Pages 148-152

DOI https://doi.org/10.1252/jcej.26.148

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Abstract

The kinetics of enzyme release from yeast using a bead mill was investigated. The enzymes investigated were invertase, acid phosphatase, alcohol dehydrogenase and alkaline phosphatase. A two-step consecutive process model was proposed to describe the behavior of enzyme release. The model described well the release of both membrane-bound and non membrane-bound enzymes, such as alkaline phosphatase and other enzymes, respectively.

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