Abstract
A plant cell culture controlling phytohormone concentration was undertaken in a bioreactor system equipped with a 2,4-dichlorophenoxyacetic acid (2,4-D)-adsorbed activated carbon column, through which the culture medium was recirculated. Elation of 2,4-D from the column was controlled by the medium recirculation rate. The elution kinetics was expressed by a simple model based on the liquid film theory.
Using this system, a 2,4-D intermittently feeding culture revealed that the 2,4-D uptake rate of Phytolacca americana callus was associated with the growth rate. In the 2,4-D constantly feeding culture, the 2,4-D concentration in the medium was maintained at an almost constant level after medium recirculation.
