JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
Online ISSN : 1881-1299
Print ISSN : 0021-9592
Research Papers
Immobilization of Lipase with Polyion Complex and Its Esterification Activity in Organic Medium
Tadahiro MurakataHiroaki MakabeYasushi YamakageKenich KobayashiHaruo FurukawaShimio Sato
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JOURNAL FREE ACCESS

1997 Volume 30 Issue 2 Pages 195-201

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Abstract

A novel method to immobilize lipase on inorganic particles or on an inorganic porous plate is proposed. The method consists of entrapment of lipase into polyion complex, followed by fixation of the complex on the inorganic supports. It was simpler and offered much saving in time, comparing with conventional entrapping and covalently binding methods. Both the lipases immobilized on the particles and the plate showed high catalytic activity for esterification in organic medium, and the activity depended on kinds of polyion complex and water contents in the immobilized-lipases. The immobilized-lipases sustained their activity up to a significantly high temperature of 60°C, which suggests that lipase and polyion complex interact to enhance thermal stability of lipase. Activity holding with repeated uses was also studied.

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© 1997 The Society of Chemical Engineers, Japan
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