Abstract
Synergistic extraction system of lactic acid is examined to develop on in situ extractive fermentation process. The addition of tri-n-butyl phosphate (TBP) to the extraction system of lactic acid (HA) with tri-n-octylamine (TOA) diluted by hexane causes a large synergism. Extraction reaction with the mixed extractant is interpreted quite well based on the formation of mixed complex, HA·TOA·2TBP. Though the addition of hexane solution containing TBP and TOA to the culture of Lactobacillus rhamnosus results in low lactate production, some lactate is produced.
