JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
Print ISSN : 0021-9592
Biochemical, Food and Medical
Clarification of Enhanced Hydroxyl Radical Production in Fenton Reaction with ATP/ADP Based on Luminol Chemiluminescence
SATOKA AOYAGIMAKOTO YAMAZAKITAKEHIRO MIYASAKAKIYOTAKA SAKAI
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2001 Volume 34 Issue 7 Pages 956-959

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Abstract

Enhanced hydroxyl radical production in the Fenton reaction with ATP or ADP based on luminol chemiluminescence was clarified. Luminol chemiluminescence intensity increases with the concentration of ATP or ADP due to their influence on the Fenton reagents which produce hydroxyl radicals. Fenton reagents containing hydrogen peroxide and ferrous ion produce hydroxyl radicals which attack luminol and this emits light. The effect of ATP and ADP on the Fenton reaction is detected as luminol CL intensity with a photon counting system. This method allows measurement of ATP or ADP concentration ranging from 0 to 15 mM, which covers the physiological concentration range.

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© 2001 The Society of Chemical Engineers, Japan
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