Submerged Culture of Agaricus blazei Mycelium in a Bubble Column Fermentor

Mikio Kawagoe; Yuki Nagaoka; Miho Araki; Keisuke Yamagaki; Kazumitsu Naoe; Hideo Noda

doi:10.1252/jcej.37.1056

Applications of Bubble Columns in Biotechnology

Submerged Culture of Agaricus blazei Mycelium in a Bubble Column Fermentor

Mikio Kawagoe, Yuki Nagaoka, Miho Araki, Keisuke Yamagaki, Kazumitsu Naoe, Hideo Noda

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Keywords: Bubble Column, Fermentor, Submerged Culture, Mushroom, Agaricus blazei

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2004 Volume 37 Issue 8 Pages 1056-1061

DOI https://doi.org/10.1252/jcej.37.1056

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Abstract

Using a bubble column fermentor, submerged culture of Agaricus blazei mycelium was carried out to find the potential for large-scale culture by bubble column fermentors. Round pellets of A. blazei mycelium of 5 mm to 20 mm in diameter were found in the fermentor. The optimum conditions on pH, cultivation temperature, compositions of nitrogen and carbon sources, and aeration rate were obtained, where the cell concentration and the overall growth rate maximum.

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