Enzymatic Oxidation of Glucose in a Pressurized Bubble Column

Abstract

The effect of pressure on the overall oxidation rate of glucose in an aqueous solution containing glucose oxidase and excess catalase was studied experimentally and theoretically in a bubble column under a pressurized condition. A bubble column with a diameter of 45 mm was used as the reactor under the pressure from 0.1 to 1.1 MPa. A model for the overall oxidation rate of glucose was presented on the basis of the film theory incorporating mass transfer of oxygen. The experimental values for the overall rate of glucose oxidation agreed approximately with the calculated values based on the present model using a previous experimental equation for the volumetric mass transfer coefficient obtained in the pressurized bubble column.