Proposal of Pumpless, Valveless, and Flowless Miniaturized Reactor Using Magnetic Beads for the Portable Analysis Device

Abstract

A miniaturized chemical-analysis system based on magnetic beads-droplet-handling mechanism without pumps and valves was developed for analysis. The analysis system has possibility for the development of a portable analysis device since there is no complicated mechanical component. In this report, we constructed an analysis system with 8 wells connected by channels. Magnetic beads with a diameter of 22.0 μm were used as a mobile phase. We demonstrated the detection of biotin-labeled DNA oligomers using this system. The result showed that this detection system has specificity and dependence of the signal on the target DNA concentration.