Abstract
The volumetric coefficients of overall mass transfer in affinity chromatography were obtained for benzamidine, trypsin, and β-galactosidase in Sepharose 4B and 6B columns. The volumetric coefficients decreased with molecular weight and with the degree of cross-linking of the adsorbents. The column productivities of the Sepharose 6B-STI column were higher than those of the Sepharose 4B-STI column because of the high operating velocity. In affinity chromatography of β-galactosidase, on the other hand, the column productivity of the Sepharose 6B-PAPTG column was extremely lower than that of the Sepharose 4B-PAPTG column.
