1986 Volume 19 Issue 1 Pages 31-36
Rate equations were derived for a two-step enzyme reaction which produced L-alanine from ammonium fumarate via L-aspartic acid by aspartase of immobilized Escherichia coli cells and L-aspartate β-decarboxylase of immobilized Pseudomonas dacunhae cells.
To establish an efficient system for industrial production of L-alanine, we investigated the design of adequate bioreactors on the basis of the simulation of this sequential reaction by solving the simultaneous equations derived.
As a result, L-alanine was found to be produced efficiently by use of two sequential column reactors: a conventional column reactor containing immobilized E. coli cells and a closed column reactor containing immobilized P. dacunhae cells.
