Abstract
The continuous separation of each protein from a colloidal solution containing several kinds of proteins was investigated by isoelectric focusing. A separation device comprising three separation cells divided by semipermeable Teflon membranes and two electrode compartments was used in this experiment. A colloidal solution containing bovine serum albumin and bovine hemoglobin was used as a sample and ampholytes were used as a buffer reagent. The costly ampholytes were recovered from effluent by ultrafiltration for reuse as buffer solution. The equation for separation efficiency was analysed semi-theoretically and the adaptability of the equation was examined experimentally. A separation efficiency of over 95 % was obtained.
