Preparation of Porous Calcium Phosphates Using a Ceramic Foaming Technique Combined with a Hydrothermal Treatment and the Cell Response with Incorporation of Osteoblast-Like Cells

Abstract

Four porous calcium phosphates (HAp, HAp/β-TCP, β-TCP, and α-TCP) were prepared using a ceramic foaming technique combined with a hydrothermal treatment. These porous calcium phosphates had enough pore size, high specific surface area, and high porosity (>80%) for the cell growth of osteoblast-like cells. The compositions of the porous calcium phosphates depended on the solution in hydrothermal treatment and the sintering temperature. The cell activities of MC3T3-E1 cells on porous HAp, HAp/β-TCP, and β-TCP were similar to that of monolayer cultivation as a control in MTT assay, ALPase activities, and the cell density.