In Vitro Crystallization of Apatite on Type I Collagen Fibrils: Role of Albumin

Abstract

Role of albumin on the biomimetic formation of calcium phosphate on collagen fibrils has been studied. Studies were done on the co-precipitation of BSA and calcium phosphate on collagen fibrils and precipitation of calcium phosphate on the collagen fibrils pre adsorbed with BSA. Appreciable changes in the morphology and crystallinity of the precipitates were observed with increase in concentration of BSA in the case of co-precipitation. The formation of spherical precipitates along the fibrils tended to change partially to a plane deposit along the fibrils. Thin film XRD and EDX study has confirmed that the precipitates formed were apatite. While the morphology of the precipitates were affected even for 1 mg/ml of BSA when pre adsorbed but crystallinity was affected gradually with increase in concentration of BSA. FT-IR study has confirmed the formation of B-type carbonated bone-like apatite and the presence of BSA in the precipitates formed from both methods.