Abstract
A new human colon cancer cell line, OUCU-LM 1, was established from a liver metastasis. OCUC-LM 1 cells proliferate in a monolayer with a population doubling time of 29.4 hours. The DNA ploidy pattern of OCUC-LM 1 was aneuploidy pattern, and the DNA index was 1.55. OCUC-LM 1 cells express various tumor associated antigens, such as CEA, CA 19-9, SLX, and SPan-1. Subcutaneous injections of OCUC-LM 1 cells induced tumor formation in nude mice, and the reconstituted tumor showed moderately differentiated adenocarcinoma. When OCUC-LM 1 cells were injected into the spleens of the nude mice, liver metastases were observed in 50% of them. On the other hand, when OCUC-LM 1 cells were injected into the spleens of the SCID mice, liver metastases were observed in all of them. Since OCUC-LM 1 cells express various carbohydrate antigens, this liver metastasis model is useful for the study of the relationship between the expression of carbohydrate antigens and the metastatic potential.
