Identification of genomic regions involved in proanthocyanidin content of black seed-coated soybean

Abstract

Proanthocyanidin (PA), contained in the seed coat of black soybeans, has positive effects on human health. There were wide genetic variations in the PA content in the black soybean seed coat. In this study, chromosomal regions associated with the PA content were determined using the segregating population developed from a cross between a low PA variety, 'Tambaguro', and a high PA variety, 'Peking'. The PA content was not related to flowering time or seed size. Single marker analysis with 150 polymorphic SSR (Single Sequence Repeat) markers revealed that Satt 592, located on Chromosome 10, was detected in both years of the experiment, while other markers were detected only in one year. These results indicate that a primary QTL (Quantitative Trait Locus) was located close to Satt 592 and that it was stable against environmental conditions.