Note
Compatibility of three bacterial strains in Agrobacterium-mediated transformation of monokaryotic mycelia of Flammulina velutipes
2018 Volume 63 Pages 31-33
Details
2018 Volume 63 Pages 31-33
Compatibility of three bacterial strains in Agrobacterium tumefaciens-mediated transformation of monokaryotic mycelia of Flammulina velutipes was compared. The bacterial strains LBA4404 and C58C1 yielded four- to five-fold higher transforming efficiencies than did EHA105. A selection medium containing 50 μg/ml of hygromycin B successfully selected the transformants.
