Development of codominant DNA markers (InDel) for Enokitake mushroom Flammulina velutipes cv. “Hatsuyuki”

Abstract

Codominant markers insertion/deletion (InDel) were developed for the Enokitake mushroom Flammulina velutipes cv. “Hatsuyuki” (HY). To detect heterozygous loci, two types of monokaryons derived from oidia (asexual spore) produced by dikaryotic mycelia HY were used. We detected heterozygous loci on a gel in 145 sets of PCR primers out of 314 sets tested. On 18 developed InDel markers randomly picked up, there was a 1:1 segregation ratio of alleles among 70 monokaryotic strains derived from basidiospores of HY, suggesting the InDel marker is available as a neutral marker under selection during spore generation and germination.