Accurate Crystal Structure Analysis
Protein Crystallography for Progress in Life Science: Charge Density Analysis of Proteins at Ultra-High Resolution
2020 Volume 62 Issue 2 Pages 106-111
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2020 Volume 62 Issue 2 Pages 106-111
The electron density distribution of each atom reflects chemical properties of the atom in the molecule. The charge-density analysis at ultra-high resolution can analyze details of the electronic distribution even in protein molecules. In this paper, we introduce a recent our result for the charge density analysis of green fluorescent protein (GFP) at an ultra-high resolution of 0.78 Å. The precise electron density distribution gives information about CH-π and lone pair-π interactions and CH…O/N type non-conventional hydrogen bonds as well as conventional hydrogen bonds. The information about such weak but substantial interactions between the chromophore and the protein environment explains the properties of GFP.
