Crystal Structure of Diol Dehydratase Reveals a New Mode of B₁₂ Binding and the Mechanism of Activation

Abstract

Diol dehydratase is an adenosylcobalamin-dependant enzyme that catalyzes conversion from 1, 2-diol compounds to corresponding aldehydes. The crystal structure of the enzyme shows that B₁₂ compounds are incorporated with the DBI-on mode. The enzyme has a potassium ion at the active site, where the substrate coordinates to it. The structure of the enzyme complexed with adeninylpentylcobalamin reveals the activation mechanism of adenosylcobalamin.