Japanese Journal of Crop Science
Online ISSN : 1349-0990
Print ISSN : 0011-1848
ISSN-L : 0011-1848
Research and Technical Note
Identification of Main Paddy Rice Cultivars in Tochigi Prefecture by RAPD Analysis
Shun-ichi KobayashiTomohiko Yoshida
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2005 Volume 74 Issue 2 Pages 207-211

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Abstract
The objective of this study was to establish the technology to identify paddy rice cultivars by Ramdom Amplified Polymorphic DNA (RAPD) analysis to raise the quality of rice in Tochigi prefecture and to prevent seed contamination in the foundation seed and the stock seed. A total of 20 paddy rice cultivars including 9 recommended and promising cultivars in Tochigi prefecture, were studied. They could be identified individually by the electrophoresis of the DNA fragments amplified by PCR using 7 random primers, on 1.5% agarose gels and by staining with ethidium bromide.
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© 2005 by The Crop Science Society of Japan
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