Abstract
Rice plants (Var. Aichiasahi) were grown in pots under 8 different nutrient conditions as follows : (1) Full nutrients (F), (2) Nitrogen only (N), (3) Phosphorus only (P), (4) Potassium only (K), (5) Without Potassium (NP), (6) Without Phosphorus (NK), (7) Without Nitrogen (PK), (8) No nutrients (O). Leaves of the definite orders were sampled from the seedling of all plots and then "heat coagulation method", which has been devised one of the authers, was applied as follows : The sampled leaves were ground out carefully and the solution were diluted by adding 30c.c. dist. water per 1gr. green weight. After filtrating it, 5c.c. solution was taken and at 60°C in the water bath. At the sametime, another 5c.c. solution was taken and added with 0.5c.c. of 10% NaCl solution as coagulater before heating as above. Then the time required for the appearance of coagulantion was measured. It was found that the coagulation generally appearance earlier in the plots which containd nitrogen such as F, N, NP, NK, than in those without nitrogen such as P, K, PK, O. Moreover, this tendency was recognized with the plants at any stages of growth as far as this experiment goes.
