On the artificial germination of pollen in rice plant

Abstract

l. As the germination beds of pollen following tbree kinds of media were Prepared : a) Agar medium : 1∼5% of agar containing 5∼30% sucrose. b) Kuzu starch medium : 10∼20% of Kuzu starch paste containing 0∼20% sucrose. c) Potato starch medium : 5∼20% of potato starch paste containing 0∼20% sucrose. Of these three kinds, the first had been popularly used by several investigators, while the last two were newly devised by the authors. 2. Pollens were collected from rice Plants (varieties : "Kyoto-Asahi" and Mokko-Mochi") when they were just in bloom. These pollens were subjected to germination tests on each beds. 3. The results showed that the germination parcentage were only 0∼3% on the agar bed, while it increased to 31∼63% on the Kuzu starch bed and to 0∼68% on the potato starch bed. 4. These results suggest that we will be able to get a satisfactory result of over 60% germination of pollen by using either 10% Kuzu starch or 5% potato strach, each containing l0∼15% sucrose.