Abstract
In this experiment, the effects of Diffusate which is assumed to contain flower promoting substance was investigated. The substauce was slowly diffused out from vernalized rye-seeds into deionized sterilized water at a cold dark room avoiding contamination of microorganism. Washed air was continuously supplied into water for four weeks until Diffusate was collected. The heading of spring rye plants when treated with Diffusate was found about two weeks earlier than that of the control plants. Spring rye was used as a tester of Diffusate-efficiency in this case. Winter rye grains were intersected at the center and were divided into two parts i. e. embryo +endosperm (part A) and endosperm (part B). Then Diffusates were collected from both A and B, and were named D-A and D-B. Three kinds of method were taken for applying D-A or D-B; (1) soaking the seed in the substance, (2) contacting a piece of cotton wet with it to the coleoptile-tip and (3) injecting it into the stems of winter rye seedlings. As shown in Fig.4, both D-A and D-B accelerated the heading of winter rye, and D-A strongly accelerateds the stem growth in lengths.
