Japanese Journal of Crop Science
Online ISSN : 1349-0990
Print ISSN : 0011-1848
ISSN-L : 0011-1848
On the Artificial Germination Test of Pollen of Barley Plant
Kiyoyuki HOZUMITakesi IGASAKI
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1957 Volume 25 Issue 3 Pages 138

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Abstract
The favorable medium for the artificial germination of pollen were studied, five varieties of barley plants being used. Concentrations of both potato starch and sucrose in the media were mainly examined. The main results were as follows :- The pollens were taken from fresh cut ears of field plants. Supply of water drop into the van Tieghem cell showed always higher percentage of germination than those without added water. The media containing potato starch in 5, 8, 10, 12, and 15 % and sucrose in 0, 5, 10, 15, and 20 % were prepared. The favorable concentrations were 5∼15 % of potato starch, each bontaining 10∼20 % sucrose. The highest germination percentage (83.7) was found in 10 % potato starch-15 % sucrose medium. The another test in which pollens were taken from plants dug out of field was conducted in comparison with the tests mentioned above. The avbrage percentage of germination was 75.2 % in the former tests and 71.4 % in the latter, but the difference was not significant.
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